Granulation is a unit operation in which small powder particles are gathered together to form agglomerates called granules. To achieve cohesion between the powders, it is necessary to include adhesive substances called binders or granulating agents within the formulation. It is a common practice to make use of a granulation solution, since it is more effective in comparison with the same quantum of the dry powder binder. Powder mixing, in conjunction with the cohesive properties of the binder, enables the formation of granules which when duly compressed using tablet press forms tablets with the desired properties.
- 1 Reasons for granulation
- 2 Ideal characteristics of granules
- 3 Manufacture of tablets by wet granulation method
- 4 Mechanisms of granule formation in wet granulation
- 5 Steps in wet granulation method of tablet production
- 5.1 Step 1: Weighing and mixing of formulation ingredients (excluding the lubricant).
- 5.2 Step 2: Preparing the damp mass
- 5.3 Step 3: Wet screening/ Screening the dampened powder into pellets or granules
- 5.4 Step 4: Drying of moist granules
- 5.5 Step 5: Sizing the granulation by dry screening
- 5.6 Step 6: Lubrication of granules
- 5.7 Step 7: Compression of granules into tablets
- 6 Recent advances in wet granulation technology
- 7 Advantages of wet granulation method of tablet manufacture
- 8 Limitations of wet granulation
- 9 Conclusion
Reasons for granulation
There are several reasons for converting powders or blends of powders into granules and they include:
- To enhance the flow properties of powder mix.
- To prevent segregation of powder components during tableting or storage.
- To reduce incidence of dust production.
- To reduce cross contamination and hazard associated with the generation of toxic dust that may arise during manufacturing processes.
- To improve the compression characteristics of drug substances.
- To improve the appearance of the final product.
Ideal characteristics of granules
For a successful manufacture of tablets, the granules must possess the following characteristics:
- All formulation ingredients should be uniformly distributed in the granules.
- A good granulation should be as near spherical in shape as possible to ensure reproducible flow which in turn ensure constant tablet weight throughout the batch.
- Granules of different sizes or density must not separate in the hopper as a result of machine vibration.
- Granules should possess good disintegrating properties and lubrication to reduce die-wall friction.
- The granules should have sufficient fines to fill empty spaces between coarse granules for better compression characteristics.
- A tablet granulation should have sufficient physical strength to form strong tablet when compacted.
Manufacture of tablets by wet granulation method
Wet granulation method is a process of size enlargement in which fine powder particles are agglomerated or brought together into larger, strong and relatively permanent structure called granules using a suitable non-toxic granulating fluid such as water, isopropanol or ethanol (or mixtures thereof). The granulating fluid can be used alone or as a solvent containing binder or granulating agent. The choice of the granulating fluid depends greatly on the properties of the materials to be granulated. Powder mixing, in conjunction with the cohesive properties of the granulating agent, enables the formation of granules. The characteristics and performance of the final product, greatly depends on the extent to which the powder particles interact with each other to form aggregates (granules).
Mechanisms of granule formation in wet granulation
Mechanisms of granule formation in wet granulation, Image source: gruppotpp
The four key mechanisms of granule formation as originally outlined by Ennis include:
1. Wetting and nucleation
This is the first and an important phase in granule formation. It involves the initial wetting of powder bed and existing granules by the granulating fluid to form nuclei. This step is largely influenced by spray rate or fluid distribution as well as feed formulation properties, in comparison with mechanical mixing. It is worth noting that the nucleation process, that is, the initial coalescence of primary particles in the immediate vicinity of the larger wetting drop is strongly linked with the wetting stage.
2. Coalescence or ball growth
In the coalescence or ball growth stage, partially wetted primary particles and larger nuclei come together to form granules composed of several particles. The more general term of coalescence refers to the successful collision of two granules to form a new, larger granule.
As granules increase in size, they are consolidated by compaction forces due to bed agitation. The extent of the consolidation depends on the agitation in the granulation equipment and the resistance of the granules to deformation. This phase in granule formation controls internal granule porosity, and therefore final properties of the granules e.g., granule strength, hardness, or dissolution.
4. Attrition or breakage
At this stage, formed granules break into fragments which bind to other granules forming a layer of material over the surviving granule.
The above mechanisms can occur simultaneously in all processes of wet granulation. However, certain mechanisms may dominate in a particular manufacturing process depending on the type of equipment used.
Steps in wet granulation method of tablet production
Wet granulation method of tablet production involves the following processing steps:
Step 1: Weighing and mixing of formulation ingredients (excluding the lubricant).
This step involves the weighing, sifting and introduction of specified quantities of drug substance(s), bulking agent, filler or diluent, and disintegrant into a powder mixer. These ingredients are mixed using either a planetary bowl mixer, ribbon/ trough mixers, rotating drum mixer or high-speed mixer until a uniform powder mix is achieved. The mixing efficiency can be enhanced by the use of powders that have similar average particle size, although this is often not the case in many mixing operations.
There are many diluents available in commerce but those used in wet granulation method include lactose, microcrystalline cellulose, starch, powdered sucrose, mannitol, fructose, sorbitol, calcium phosphate and calcium sulphate. Among these diluents the most widely used are lactose, because of its low cost, solubility and compatibility with most drug substances and excipients and microcrystalline cellulose, because of its easy compaction, compatibility with most formulation ingredients and consistent uniformity of supply. Diluents are usually selected based on the manufacturer’s experience with the material, its relative cost, and its compatibility with the drug and other excipients.
Disintegrants used in wet granulation include croscarmellose, sodium starch glycolate, sodium carboxymethylcellulose, polyvinylpyrrolidone (PVP), crospovidone, cation exchange resins, corn and potato starches, alginic acid and other materials that counteract the effect of binders and the physical forces of compression used in forming the tablets. Croscarmellose (2%) and sodium starch glycolate (5%) are often used because of their high water uptake and rapid action.
Step 2: Preparing the damp mass
Here, the binder solution is mixed with the powder mixture to form an adhesive mass which can be granulated. The amount of binding agent used as well as the quantity of fluid required to form a damp and coherent mass is part of the operator’s skill; however, the resulting binder-powder mixture should compact when squeezed in the hand. The use of insufficient binder tends to poor adhesion, capping and soft tablets. Excessive binder solution yields hard tablets with slow disintegrating properties.
Among granulating agents are solutions of povidone, an aqueous preparation of cornstarch, molasses, methylcellulose, carboxymethylcellulose, glucose solution and microcrystalline cellulose.
Dry binder or nonaqueous solution may be used for drug substances that are adversely affected by aqueous solution. Colorants or flavouring agents may be added to the binding agent to prepare a granulation with an added feature.
Step 3: Wet screening/ Screening the dampened powder into pellets or granules
The wet massed powder blend is screened using 6- to 12- mesh screen to prepare wet granules. This may be done by hand or with suitable equipment that prepares the granules by extrusion through perforations in the apparatus. The granules formed are spread evenly on trays and dried in an oven.
Step 4: Drying of moist granules
The screened moist granules are dried in an oven at a controlled temperature not exceeding 550C to a consistent weight or constant moisture content. The drying temperature and the duration of drying process depend on the nature of the active ingredient and the level of moisture required for the successful production of satisfactory tablets. Shelf or tray drier and fluidized-bed drier can be used for this purpose.
Step 5: Sizing the granulation by dry screening
The dried granules are passed through a screen of smaller size than that used to prepare the moist granules. The size of the final granules is dependent on the size of the punches (and hence the final tablet size). Screens of 14- to 20- mesh size are generally used for this purpose.
Step 6: Lubrication of granules
After dry screening, the dried and screened granules are separated into coarse and fine granules by shaking them on a 250 mesh sieve. Appropriate quantity of lubricant is passed through a 200 – mesh sieve. This is mixed with the fine granules before the coarse granules are incorporated. The quantity of lubricant used varies from one formulation scientist to another but usually ranges from about 0.1% to 5% of the weight of the granulation.
Examples of lubricants commonly used in wet granulation include magnesium stearate (most preferred), calcium stearate, stearic acid, wax, hydrogenated vegetable oil, talc, and starch.
It is worth noting that disintegrant may be added in step 1 (intragranular) or in step 6 (extragranular) and sometimes in both steps (intragranular – extragranular). Intragranular – extragranular incorporation appears to be the best method of incorporation because the extragranularly added portion causes immediate disruption of the tablet into the previously compressed granules while the portion added intragranuarly cause further erosion of the granules to the original powder particles.
Step 7: Compression of granules into tablets
Here, the mixed granules are compressed in a single punch or multi-station tablet press fitted with the appropriate punches and dies.
Compressed tablets may be coated if there is need to mask the taste of unpleasant drugs, increase the aesthetic appeal of uncoated tablets, modify or control the release of therapeutic agents from tablets. This is achieved by enclosing or covering the core tablet or granules with coating solutions.
Recent advances in wet granulation technology
In an urge to improve commercial output of pharmaceutical formulations, wet granulation process has witnessed various technical and technological innovations such as
- Steam granulation
- Moisture-Activated Dry Granulation/ moist granulation
- Freeze granulation
- Thermal adhesion granulation
- Melt granulation/ thermoplastic granulation
- Foam granulation and
- Reverse wet granulation/ reverse-phase wet granulation
Advantages of wet granulation method of tablet manufacture
- Wet granulation modifies the properties of formulation ingredients to overcome their tableting deficiencies. Granules formed are relatively more spherical than the powders and have better flow properties.
- Improved compressibility of powders resulting from wet granulation process allows the use of low pressure during compression. This reduces machine wear and thus improves the life of the machine.
- The process makes use of conventional excipients and therefore is not dependent on the inclusion of special grades of excipients.
- It ensures better content uniformity, especially for soluble low-dose drugs.
- The process may improve the dissolution rate of poorly soluble drugs by imparting hydrophilic properties to the surface of the granules.
- Wet granulation prevents segregation of components of a homogenous powder mix during processing, transferring, handling and/or storage, leading to reduced intra- and inter-batch variability.
- Tablets manufactured by wet granulation are amenable to post-processing unit operations such as tablet coating.
- Wet granulation reduces the level of dust present during manufacturing process thereby reducing the incidence of cross contamination and risk to workers.
- Wet granulation reduces the amount of air entrapment thereby increasing powder compressibility.
Limitations of wet granulation
- Wet granulation often require several processing steps.
- The cost of wet granulation is higher because of the time, labour, energy, equipment and space required for the process.
- The process is not suitable for thermolabile and moisture sensitive materials.
- Migration of soluble dyes may occur during the drying process.
- Incompatibilities between formulation ingredients will be aggravated by the granulating solvent which tends to bring them into close contact.
- There is a possibility of material loss during processing due to the transfer of material from one unit operation to the other.
- Dissolution rate of tablets manufactured by wet granulation may decrease with aging.
In spite of all these limitations, the manufacture of tablet using wet granulation still persist due to the following reasons:
- Availability of extensive data on manufacture of existing products by wet granulation. This has made pharmaceutical companies reluctant to adopt new techniques except there exist a compelling reason to do that.
- Experience over the years in formulation has also shown that wet granulated granules and tablets assure good content uniformity.
- The drying process can be manipulated to produce granules with the desired moisture content.
The manufacture of tablets by wet granulation involves several unit operations. In order to manufacture tablets with desired characteristics, it is important to have a good understanding of the processes involved.
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